A framework for variation discovery and genotyping using next-generation DNA sequencing data.
Recent advances in sequencing technology make it possible to comprehensively catalog genetic variation in population samples, creating a foundation for understanding human disease, ancestry and evolution. The amounts of raw data produced are prodigious, and many computational steps are required to translate this output into high-quality variant calls. We present a unified analytic framework to discover and genotype variation among multiple samples simultaneously that achieves sensitive and specific results across five sequencing technologies and three distinct, canonical experimental designs.
Our process includes (i) initial read mapping; (ii) local realignment around indels; (iii) base quality score recalibration; (iv) SNP discovery and genotyping to find all potential variants; and (v) machine learning to separate true segregating variation from machine artifacts common to next-generation sequencing technologies. We here discuss the application of these tools, instantiated in the Genome Analysis Toolkit, to deep whole-genome, whole-exome capture and multi-sample low-pass (∼4×) 1000 Genomes Project datasets.
Match cell/lid QS LP10mm 1.4ml |
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| CEL2120 | Scientific Laboratory Supplies | 1PR | EUR 343.37 |
Match cell/stpr QS/GLP10mm 3.5ml |
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| CEL2052 | Scientific Laboratory Supplies | 1PR | EUR 454.35 |
Total RNA - Mouse Normal Tissue: Brain |
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| R1334035-50 | Biochain | 50 ug | EUR 130 |
Rat Creatine kinase for muscle and brain (CKMB) |
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| QY-E11888 | Qayee Biotechnology | 96T | EUR 448.8 |
Genomic DNA - Mouse Normal Tissue: Brain |
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| D1334035 | Biochain | 100 ug | EUR 286 |
Total RNA - Human Adult Normal Tissue: Brain |
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| R1234035-50 | Biochain | 50 ug | EUR 170 |
Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Lung |
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| D8235152-PP-10 | Biochain | 2x10 ug | EUR 416 |
Total RNA - Monkey (Rhesus) Normal Tissue: Brain |
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| R1534035-50 | Biochain | 50 ug | EUR 191 |
Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Colon |
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| D8235090-PP-10 | Biochain | 2x10 ug | EUR 416 |
Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Liver |
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| D8235149-PP-10 | Biochain | 2x10 ug | EUR 416 |
Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Lung |
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| R8235152-PP-10 | Biochain | 2x10 ug | EUR 463 |
Brain Dissociation System 7 (Endothelial), Mouse and Rat |
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| 4-20227 | CHI Scientific | ea | Ask for price |
Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Breast |
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| D8235086-PP-10 | Biochain | 2x10 ug | EUR 416 |
Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Kidney |
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| D8235142-PP-10 | Biochain | 2x10 ug | EUR 416 |
Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Rectum |
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| D8235206-PP-10 | Biochain | 2x10 ug | EUR 416 |
Brain and Acute Leukemia, Cytoplamatic |
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| PR27238 | Neuromics | 2 ug | EUR 229.2 |
Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Colon |
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| R8235090-PP-10 | Biochain | 2x10 ug | EUR 463 |
Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Liver |
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| R8235149-PP-10 | Biochain | 2x10 ug | EUR 463 |
Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Stomach |
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| D8235248-PP-10 | Biochain | 2x10 ug | EUR 416 |
Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Breast |
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| R8235086-PP-10 | Biochain | 2x10 ug | EUR 463 |
Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Kidney |
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| R8235142-PP-10 | Biochain | 2x10 ug | EUR 463 |
Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Rectum |
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| R8235206-PP-10 | Biochain | 2x10 ug | EUR 463 |
Total RNA - Monkey (Cynomolgus) Normal Tissue: Brain |
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| R1534035-Cy | Biochain | 50 ug | EUR 191 |
Matched Pair - DNA - Human Primary and Metastatic Tumor Tissue: Lung |
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| D8235152-PM-10 | Biochain | 2x10 ug | EUR 690 |
Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Stomach |
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| R8235248-PP-10 | Biochain | 2x10 ug | EUR 463 |
Matched Pair - DNA - Human Primary and Metastatic Tumor Tissue: Colon |
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| D8235090-PM-10 | Biochain | 2x10 ug | EUR 690 |
Matched Pair - DNA - Human Primary and Metastatic Tumor Tissue: Liver |
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| D8235149-PM-10 | Biochain | 2x10 ug | EUR 690 |
Brain tumor and brain tissue array(2016 WHO CNS tumor classification) |
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| GL208a | TissueArray | each | EUR 546 |
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Description: Brain tumor and brain tissue array(2016 WHO CNS tumor classification), including pathology grade, 69 cases/207 cores (cores size 1.0mm), replacing GL208 |
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Matched Pair - Total RNA - Human Primary and Metastatic Tumor Tissue: Lung |
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| R8235152-PM-10 | Biochain | 2x10 ug | EUR 716 |
Matched Pair - DNA - Human Primary and Metastatic Tumor Tissue: Breast |
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| D8235086-PM-10 | Biochain | 2x10 ug | EUR 690 |
Matched Pair - DNA - Human Primary and Metastatic Tumor Tissue: Kidney |
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| D8235142-PM-10 | Biochain | 2x10 ug | EUR 690 |
Matched Pair - DNA - Human Primary and Metastatic Tumor Tissue: Rectum |
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| D8235206-PM-10 | Biochain | 2x10 ug | EUR 690 |
Brain tumor and normal tissue array |
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| GL1002 | TissueArray | each | EUR 306 |
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Description: Brain tumor and normal tissue array, 50 cases/100 cores |
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Brain tumor and normal tissue array |
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| GL1201 | TissueArray | each | EUR 330 |
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Description: Brain tumor and normal tissue array, including Meningioma and Astrocytoma, Oligodendroglioma, Glioblastoma, Neurilemmoma, Medulloblastoma, Ependymoma, lymphoma, Choroids plexus papilloma, Pituitary adenoma, Germ cell tumours, Acoustic neuroma, craniopharyngioma, Haemangioblastoma, Primal neuroectodermal tumor, with pathology grade, 120 cases/120 cores (core size 1.5mm),replacing BC17013 |
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Brain tumor and normal tissue array |
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| GL721 | TissueArray | each | EUR 306 |
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Description: Brain tumor and normal tissue array, including pathology grade, 24 cases/72 cores |
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FFPE and Frozen Matched Pair Genomic DNA: Human Tumor Tissue: Lung |
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| D8235152-FP | Biochain | 2 x 2 ug | EUR 2374 |
Matched Pair - Total RNA - Human Primary and Metastatic Tumor Tissue: Colon |
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| R8235090-PM-10 | Biochain | 2x10 ug | EUR 716 |
Matched Pair - Total RNA - Human Primary and Metastatic Tumor Tissue: Liver |
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| R8235149-PM-10 | Biochain | 2x10 ug | EUR 716 |
Matched Pair - DNA - Human Primary and Metastatic Tumor Tissue: Stomach |
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| D8235248-PM-10 | Biochain | 2x10 ug | EUR 690 |
Brain cancer and normal tissue array |
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| GL1001a | TissueArray | each | EUR 306 |
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Description: Brain cancer and normal tissue array, including pathology grade, 100 cases/100 cores, replacing GL1001 |
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FFPE and Frozen Matched Pair Genomic DNA: Human Tumor Tissue: Colon |
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| D8235090-FP | Biochain | 2 x 2 ug | EUR 2374 |
Matched Pair - Total RNA - Human Primary and Metastatic Tumor Tissue: Breast |
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| R8235086-PM-10 | Biochain | 2x10 ug | EUR 716 |
Matched Pair - Total RNA - Human Primary and Metastatic Tumor Tissue: Kidney |
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| R8235142-PM-10 | Biochain | 2x10 ug | EUR 716 |
Matched Pair - Total RNA - Human Primary and Metastatic Tumor Tissue: Rectum |
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| R8235206-PM-10 | Biochain | 2x10 ug | EUR 716 |
Total RNA - Human Adult Normal Tissue: Brain: Pons |
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| R1234071-10 | Biochain | 10 ug | EUR 183 |
FFPE and Frozen Matched Pair Total RNA: Human Adult Normal Tissue: Liver |
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| R8234149-FP | Biochain | 2x1 ug | EUR 1664 |
Brain Dissociation System 2 (Glial, Schwann), Mouse and Rat |
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| 4-20222 | CHI Scientific | ea | Ask for price |
FFPE and Frozen Matched Pair Genomic DNA: Human Tumor Tissue: Breast |
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| D8235086-FP | Biochain | 2 x 2 ug | EUR 2374 |
Matched Pair - Total RNA - Human Primary and Metastatic Tumor Tissue: Stomach |
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| R8235248-PM-10 | Biochain | 2x10 ug | EUR 716 |
FFPE and Frozen Matched Pair Genomic DNA: Human Tumor Tissue: Stomach |
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| D8235248-FP | Biochain | 2 x 2 ug | EUR 2520 |
Brain Dissociation System 5 (Cerebral cortex), Mouse and Rat |
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| 4-20225 | CHI Scientific | ea | Ask for price |
Kidney And Brain Protein (KIBRA) Antibody |
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| 20-abx177265 | Abbexa |
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Kidney And Brain Protein (KIBRA) Antibody |
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| 20-abx131689 | Abbexa |
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Total RNA - Human Adult Normal Tissue: Brain: Thalamus |
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| R1234079-50 | Biochain | 50 ug | EUR 170 |
Recombinant Kidney And Brain Protein (KIBRA) |
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| 4-RPL824Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Kidney And Brain Protein expressed in: E.coli |
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Total RNA - Human Adult Normal Tissue: Brain: Cerebellum |
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| R1234039-50 | Biochain | 50 ug | EUR 170 |
Rat Brain PrimaCell1: Normal Brain Fibroblasts |
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| 2-82511 | CHI Scientific | 1 Kit | Ask for price |
Brain Dissociation System 3 (Hippocampal neurons), Mouse and Rat |
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| 4-20223 | CHI Scientific | ea | Ask for price |
Baalc ELISA Kit| Rat Brain and acute leukemia cytoplasmic prote |
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| EF018386 | Lifescience Market | 96 Tests | EUR 826.8 |
Total RNA - Human Adult Normal Tissue: Brain: Hippocampus |
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| R1234052-10 | Biochain | 10 ug | EUR 643 |
Total RNA - Human Adult Normal Tissue: Brain: Diencephalon |
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| R1234049-10 | Biochain | 10 ug | EUR 183 |
Brain astrocytoma and meningioma tissue array |
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| BC17013 | TissueArray | each | EUR 306 |
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Description: Brain astrocytoma and meningioma tissue array, 120 cases/120 cores |
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Rat Brain And Acute Leukemia Cytoplasmic Protein (BAALC) ELISA Kit |
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| abx391033-96tests | Abbexa | 96 tests | EUR 1093.2 |
Brain and Acute Leukemia, Cytoplasmatic Protein |
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| 20-abx262869 | Abbexa |
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Brain Dissociation System 4 (Endothelial, Microvessels), Mouse and Rat |
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| 4-20224 | CHI Scientific | ea | Ask for price |
FFPE and Frozen Matched Pair Genomic DNA: Human Adult Normal Tissue: Liver |
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| D8234149-FP | Biochain | 2 x 2 ug | EUR 2099 |
Total RNA - Human Adult Normal Tissue: Brain: Frontal Lobe |
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| R1234051-50 | Biochain | 50 ug | EUR 170 |
Mouse Kidney And Brain Protein (KIBRA) Protein |
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| 20-abx650801 | Abbexa |
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Total RNA - Human Adult Normal Tissue: Brain: Parietal Lobe |
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| R1234066-50 | Biochain | 50 ug | EUR 170 |
Total RNA - Human Adult Normal Tissue: Brain: Temporal Lobe |
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| R1234078-50 | Biochain | 50 ug | EUR 170 |
Brain Dissociation System 6 (Brain), Fetal Rat |
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| 4-20226 | CHI Scientific | ea | Ask for price |
Total RNA - Human Adult Normal Tissue: Brain: Occipital Lobe |
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| R1234062-50 | Biochain | 50 ug | EUR 170 |
Total RNA - Human Adult Normal Tissue: Brain: Cerebellum (left) |
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| R1234040-10 | Biochain | 10 ug | EUR 183 |
Total RNA - Human Adult Normal Tissue: Brain: Cerebral Cortex |
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| R1234042-10 | Biochain | 10 ug | EUR 183 |
Total RNA - Human Adult Normal Tissue: Brain: Corpus Callosum |
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| R1234045-10 | Biochain | 10 ug | EUR 183 |
Total RNA - Human Adult Normal Tissue: Brain: Cerebellum (right) |
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| R1234041-10 | Biochain | 10 ug | EUR 183 |
Total RNA - Human Adult Normal Tissue: Brain: Olfactory (region) |
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| R1234063-10 | Biochain | 10 ug | EUR 286 |
Total RNA - Human Adult Normal Tissue: Brain: Precentral Gyrus |
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| R1234073-10 | Biochain | 10 ug | EUR 183 |
Total RNA - Human Adult Normal Tissue: Brain: Cerebral Meninges |
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| R1234043-10 | Biochain | 10 ug | EUR 183 |
Total RNA - Human Adult Normal Tissue: Brain: Medulla oblongata |
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| R1234057-50 | Biochain | 50 ug | EUR 170 |
Total RNA - Human Adult Normal Tissue: Brain: Postcentral Gyrus |
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| R1234072-10 | Biochain | 10 ug | EUR 183 |
Pituitary And Brain Cadherin (PB Cadherin) Antibody |
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| 20-abx133389 | Abbexa |
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Total RNA - Human Adult Normal Tissue 5 Donor Pool: Brain |
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| R1234035-P | Biochain | 50 ug | EUR 370 |
Mouse Kidney And Brain Protein (KIBRA) CLIA Kit |
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| 20-abx495976 | Abbexa |
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Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| 20-abx154290 | Abbexa |
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Brain And Muscle ARNT-Like 1 (BMAL1) Antibody |
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| abx148343-100ug | Abbexa | 100 ug | EUR 526.8 |
Brain And Muscle ARNT-Like 1 (BMAL1) Antibody |
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| abx430327-200ul | Abbexa | 200 ul | EUR 460.8 |
Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| DLR-KIBRA-Mu-48T | DL Develop | 48T | EUR 679.2 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Kidney And Brain Protein (KIBRA) in samples from tissue homogenates, cell lysates or other biological fluids. |
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Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| DLR-KIBRA-Mu-96T | DL Develop | 96T | EUR 889.2 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Kidney And Brain Protein (KIBRA) in samples from tissue homogenates, cell lysates or other biological fluids. |
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Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| SEL824Mu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 6400.37 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Kidney And Brain Protein (KIBRA) in Tissue homogenates, cell lysates and other biological fluids. |
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Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| SEL824Mu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 632.27 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Kidney And Brain Protein (KIBRA) in Tissue homogenates, cell lysates and other biological fluids. |
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Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| SEL824Mu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 851.81 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Kidney And Brain Protein (KIBRA) in Tissue homogenates, cell lysates and other biological fluids. |
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Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| SEL824Mu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3473.14 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Kidney And Brain Protein (KIBRA) in Tissue homogenates, cell lysates and other biological fluids. |
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Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| 4-SEL824Mu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Kidney And Brain Protein (KIBRA) in samples from Tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species. |
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Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| RDR-KIBRA-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 723.6 |
Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| RDR-KIBRA-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 1008 |
Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| RD-KIBRA-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 692.4 |
Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| RD-KIBRA-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 962.4 |
Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| RD-KIBRA-Mu-48T | Reddot Biotech | 48T | EUR 506.3 |
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Description: tissue homogenates, cell lysates and other biological fluids. |
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Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| RD-KIBRA-Mu-96T | Reddot Biotech | 96T | EUR 723.3 |
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Description: tissue homogenates, cell lysates and other biological fluids. |
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Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| RDR-KIBRA-Mu-48T | Reddot Biotech | 48T | EUR 531.62 |
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Description: tissue homogenates, cell lysates and other biological fluids. |
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Mouse Kidney And Brain Protein (KIBRA) ELISA Kit |
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| RDR-KIBRA-Mu-96T | Reddot Biotech | 96T | EUR 759.47 |
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Description: tissue homogenates, cell lysates and other biological fluids. |
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Brain Dissociation System 8 (Capillary endothelium, Pericytes), Mouse and Rat |
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| 4-20228 | CHI Scientific | ea | Ask for price |
Genomic DNA - Dementia: Brain: Thalamus, from a single donor |
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| D1236079Dem | Biochain | 50 ug | EUR 489 |
Genomic DNA - Alzheimer's Disease: Brain, from a single donor |
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| D1236035Alz | Biochain | 50 ug | EUR 489 |
Genomic DNA - Parkinson's Disease: Brain, from a single donor |
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| D1236035Par | Biochain | 50 ug | EUR 489 |
Rat Brain PrimaCell1: Normal Brain Fibroblasts Growth Medium |
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| 9-25011 | CHI Scientific | 5 x 100 ml | Ask for price |
Creatine Kinase, Muscle and Brain (CKM/CKB) Antibody |
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| abx411275-01mg | Abbexa | 0.1 mg | EUR 526.8 |
Recombinant Human Brain and Acute Leukemia, Cytoplasmatic |
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| 7-04471 | CHI Scientific | 2µg | Ask for price |
Recombinant Human Brain and Acute Leukemia, Cytoplasmatic |
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| 7-04472 | CHI Scientific | 10µg | Ask for price |
Recombinant Human Brain and Acute Leukemia, Cytoplasmatic |
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| 7-04473 | CHI Scientific | 1mg | Ask for price |
Genomic DNA - Depression: Brain: Thalamus, from a single donor |
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| D1236079Dep | Biochain | 50 ug | EUR 489 |
Kin17 DNA And RNA Binding Protein (KIN) Protein |
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| 20-abx260971 | Abbexa |
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Kin17 DNA And RNA Binding Protein (KIN) Antibody |
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| abx234575-100ug | Abbexa | 100 ug | EUR 610.8 |
Kin17 DNA And RNA Binding Protein (KIN) Antibody |
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| 20-abx113374 | Abbexa |
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Rat Brain Tissue Lysate |
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| LYSATE0002 | BosterBio | 200ug | EUR 180 |
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Description: This cell lysate is prepared from Rat Brain Tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
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Rat Brain Tissue Preparation Buffer 1: Normal Brain Fibroblasts |
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| 9-80218 | CHI Scientific | 1 x 100 ml | Ask for price |
ELISA kit for Mouse KIBRA (Kidney And Brain Protein) |
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| ELK8022 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
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Description: A sandwich ELISA kit for detection of Kidney And Brain Protein from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
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Kidney And Brain Protein (KIBRA) Polyclonal Antibody (Mouse) |
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| 4-PAL824Mu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Kidney And Brain Protein (KIBRA) |
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Genomic DNA - Dementia: Brain: Frontal Lobe, from a single donor |
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| D1236051Dem | Biochain | 50 ug | EUR 489 |
Total RNA - Human Adult Normal Tissue: Brain, Ambient temperature shipping |
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| ATR1234035-50 | Biochain | 50 ug | EUR 185 |
Genomic DNA - Dementia: Brain: Parietal Lobe, from a single donor |
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| D1236066Dem | Biochain | 50 ug | EUR 489 |
Genomic DNA - Alzheimer's Disease: Brain: Pons, from a single donor |
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| D1236071Alz | Biochain | 50 ug | EUR 489 |
Genomic DNA - Parkinson's Disease: Brain: Pons, from a single donor |
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| D1236071Par | Biochain | 50 ug | EUR 489 |
Genomic DNA - Dementia: Brain: Temporal Lobe, from a single donor |
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| D1236078Dem | Biochain | 50 ug | EUR 489 |
The 1982 revised criteria for the classification of systemic lupus erythematosus
The 1971 preliminary criteria for the classification of systemic lupus erythematosus (SLE) were revised and updated to incorporate new immunologic knowledge and improve disease classification. The 1982 revised criteria include fluorescence antinuclear antibody and antibody to native DNA and Sm antigen. Some criteria involving the same organ systems were aggregated into single criteria.
Raynaud’s phenomenon and alopecia were not included in the 1982 revised criteria because of low sensitivity and specificity. The new criteria were 96% sensitive and 96% specific when tested with SLE and control patient data gathered from 18 participating clinics. When compared with the 1971 criteria, the 1982 revised criteria showed gains in sensitivity and specificity.
Translating the histone code
Chromatin, the physiological template of all eukaryotic genetic information, is subject to a diverse array of posttranslational modifications that largely impinge on histone amino termini, thereby regulating access to the underlying DNA. Distinct histone amino-terminal modifications can generate synergistic or antagonistic interaction affinities for chromatin-associated proteins, which in turn dictate dynamic transitions between transcriptionally active or transcriptionally silent chromatin states.
The combinatorial nature of histone amino-terminal modifications thus reveals a “histone code” that considerably extends the information potential of the genetic code. We propose that this epigenetic marking system represents a fundamental regulatory mechanism that has an impact on most, if not all, chromatin-templated processes, with far-reaching consequences for cell fate decisions and both normal and pathological development.
Genomic sequencing
Unique DNA sequences can be determined directly from mouse genomic DNA. A denaturing gel separates by size mixtures of unlabeled DNA fragments from complete restriction and partial chemical cleavages of the entire genome. These lanes of DNA are transferred and UV-crosslinked to nylon membranes. Hybridization with a short 32P-labeled single-stranded probe produces the image of a DNA sequence “ladder” extending from the 3′ or 5′ end of one restriction site in the genome. Numerous different sequences can be obtained from a single membrane by reprobing.
Each band in these sequences represents 3 fg of DNA complementary to the probe. Sequence data from mouse immunoglobulin heavy chain genes from several cell types are presented. The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
A new method for sequencing DNA
DNA can be sequenced by a chemical procedure that breaks a terminally labeled DNA molecule partially at each repetition of a base. The lengths of the labeled fragments then identify the positions of that base. We describe reactions that cleave DNA preferentially at guanines, at adenines, at cytosines and thymines equally, and at cytosines alone.
When the products of these four reactions are resolved by size, by electrophoresis on a polyacrylamide gel, the DNA sequence can be read from the pattern of radioactive bands. The technique will permit sequencing of at least 100 bases from the point of labeling.
Genome sequencing in microfabricated high-density picolitre reactors
The proliferation of large-scale DNA-sequencing projects in recent years has driven a search for alternative methods to reduce time and cost. Here we describe a scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments. The apparatus uses a novel fibre-optic slide of individual wells and is able to sequence 25 million bases, at 99% or better accuracy, in one four-hour run.
To achieve an approximately 100-fold increase in throughput over current Sanger sequencing technology, we have developed an emulsion method for DNA amplification and an instrument for sequencing by synthesis using a pyrosequencing protocol optimized for solid support and picolitre-scale volumes. Here we show the utility, throughput, accuracy and robustness of this system by shotgun sequencing and de novo assembly of the Mycoplasma genitalium genome with 96% coverage at 99.96% accuracy in one run of the machine.
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers
A series of plasmid vectors containing the multiple cloning site (MCS7) of M13mp7 has been constructed. In one of these vectors a kanamycin-resistance marker has been inserted into the center of the symmetrical MCS7 to yield a restriction-site-mobilizing element (RSM).
The drug-resistance marker can be cleaved out of this vector with any of the restriction enzymes that recognize a site of the flanking sequences of the RSM to generate an RSM with either various sticky ends or blunt ends. These fragments can be used for insertion mutagenesis of any target molecule with compatible restriction sites. Insertion mutants are selected by their resistance to kanamycin.
When the drug-resistance marker is removed with PstI, a small in-frame insertion can be generated. In addition, two new MCSs having single restriction sites have been formed by altering the symmetrical structure of MCS7. The resulting plasmids pUC8 and pUC9 allow one to clone doubly digested restriction fragments separately with both orientations in respect to the lac promoter. The terminal sequences of any DNA cloned in these plasmids can be characterized using the universal M13 primers.
DNAJB14 Polyclonal Antibody |
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| A66234 | EpiGentek |
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DNAJB14 Antibody, HRP conjugated |
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| 1-CSB-PA819454LB01HU | Cusabio |
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Description: A polyclonal antibody against DNAJB14. Recognizes DNAJB14 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
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DNAJB14 Antibody, FITC conjugated |
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| 1-CSB-PA819454LC01HU | Cusabio |
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Description: A polyclonal antibody against DNAJB14. Recognizes DNAJB14 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
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DNAJB14 Antibody, Biotin conjugated |
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| 1-CSB-PA819454LD01HU | Cusabio |
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Description: A polyclonal antibody against DNAJB14. Recognizes DNAJB14 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
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DNAJB14 Polyclonal Conjugated Antibody |
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| C30628 | SAB | 100ul | EUR 476.4 |
DNAJB14 Polyclonal Antibody, HRP Conjugated |
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| A66235 | EpiGentek |
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DNAJB14 Polyclonal Antibody, FITC Conjugated |
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| A66236 | EpiGentek |
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DNAJB14 Polyclonal Antibody, Biotin Conjugated |
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| A66237 | EpiGentek |
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DnaJ (Hsp40) Homolog, Subfamily B, Member 14 (DNAJB14) Antibody |
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| abx232446-100ug | Abbexa | 100 ug | EUR 577.2 |
DnaJ (Hsp40) Homolog, Subfamily B, Member 14 (DNAJB14) Antibody |
|||
| 20-abx112129 | Abbexa |
|
|
DnaJ (Hsp40) Homolog, Subfamily B, Member 14 (DNAJB14) Antibody |
|||
| 20-abx003806 | Abbexa |
|
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DnaJ (Hsp40) Homolog, Subfamily B, Member 14 (DNAJB14) Antibody |
|||
| 20-abx302018 | Abbexa |
|
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DnaJ (Hsp40) Homolog, Subfamily B, Member 14 (DNAJB14) Antibody (HRP) |
|||
| 20-abx308795 | Abbexa |
|
|
DnaJ (Hsp40) Homolog, Subfamily B, Member 14 (DNAJB14) Antibody (FITC) |
|||
| 20-abx308796 | Abbexa |
|
|
DnaJ (Hsp40) Homolog, Subfamily B, Member 14 (DNAJB14) Antibody (Biotin) |
|||
| 20-abx308797 | Abbexa |
|
|
DNAJB14 siRNA |
|||
| 20-abx914343 | Abbexa |
|
|
DNAJB14 siRNA |
|||
| 20-abx914344 | Abbexa |
|
|
DNAJB14 Rabbit pAb |
|||
| A4990-100ul | Abclonal | 100 ul | EUR 369.6 |
DNAJB14 Rabbit pAb |
|||
| A4990-200ul | Abclonal | 200 ul | EUR 550.8 |
DNAJB14 Rabbit pAb |
|||
| A4990-20ul | Abclonal | 20 ul | EUR 219.6 |
DNAJB14 Rabbit pAb |
|||
| A4990-50ul | Abclonal | 50 ul | EUR 267.6 |
DNAJB14 cloning plasmid |
|||
| CSB-CL819454HU-10ug | Cusabio | 10ug | EUR 279.6 |
|
Description: A cloning plasmid for the DNAJB14 gene. |
|||
DNAJB14 Blocking Peptide |
|||
| DF12198-BP | Affbiotech | 1mg | EUR 234 |
anti- DNAJB11 antibody |
|||
| FNab02443 | FN Test | 100µg | EUR 606.3 |
|
Description: Antibody raised against DNAJB11 |
|||
anti- DNAJB12 antibody |
|||
| FNab02444 | FN Test | 100µg | EUR 658.5 |
|
Description: Antibody raised against DNAJB12 |
|||
anti- DNAJB13 antibody |
|||
| FNab02445 | FN Test | 100µg | EUR 702 |
|
Description: Antibody raised against DNAJB13 |
|||
DNAJB14 ELISA KIT|Human |
|||
| EF009156 | Lifescience Market | 96 Tests | EUR 826.8 |
Mouse DNAJB14 shRNA Plasmid |
|||
| 20-abx977260 | Abbexa |
|
|
Human DNAJB14 shRNA Plasmid |
|||
| 20-abx962706 | Abbexa |
|
|
anti- DNAJB1 antibody |
|||
| FNab02442 | FN Test | 100µg | EUR 606.3 |
|
Description: Antibody raised against DNAJB1 |
|||
anti- DNAJB2 antibody |
|||
| FNab02447 | FN Test | 100µg | EUR 606.3 |
|
Description: Antibody raised against DNAJB2 |
|||
anti- DNAJB2 antibody |
|||
| FNab02448 | FN Test | 100µg | EUR 658.5 |
|
Description: Antibody raised against DNAJB2 |
|||
anti- DNAJB3 antibody |
|||
| FNab02449 | FN Test | 100µg | EUR 658.5 |
|
Description: Antibody raised against DNAJB3 |
|||
anti- DNAJB4 antibody |
|||
| FNab02450 | FN Test | 100µg | EUR 606.3 |
|
Description: Antibody raised against DNAJB4 |
|||
anti- DNAJB5 antibody |
|||
| FNab02451 | FN Test | 100µg | EUR 606.3 |
|
Description: Antibody raised against DNAJB5 |
|||
anti- DNAJB6 antibody |
|||
| FNab02452 | FN Test | 100µg | EUR 606.3 |
|
Description: Antibody raised against DNAJB6 |
|||
anti- DNAJB7 antibody |
|||
| FNab02453 | FN Test | 100µg | EUR 606.3 |
|
Description: Antibody raised against DNAJB7 |
|||
anti- DNAJB8 antibody |
|||
| FNab02454 | FN Test | 100µg | EUR 658.5 |
|
Description: Antibody raised against DNAJB8 |
|||
anti- DNAJB9 antibody |
|||
| FNab02455 | FN Test | 100µg | EUR 606.3 |
|
Description: Antibody raised against DNAJB9 |
|||
DNAJB14 Recombinant Protein (Rat) |
|||
| RP198299 | ABM | 100 ug | Ask for price |
DNAJB14 Recombinant Protein (Human) |
|||
| RP009529 | ABM | 100 ug | Ask for price |
DNAJB14 Recombinant Protein (Mouse) |
|||
| RP129476 | ABM | 100 ug | Ask for price |
Dnajb14 ORF Vector (Rat) (pORF) |
|||
| ORF066101 | ABM | 1.0 ug DNA | EUR 607.2 |
DNAJB14 ORF Vector (Human) (pORF) |
|||
| ORF003177 | ABM | 1.0 ug DNA | EUR 114 |
Dnajb14 ORF Vector (Mouse) (pORF) |
|||
| ORF043160 | ABM | 1.0 ug DNA | EUR 607.2 |
Anti-MDG1 / DNAJB9 antibody |
|||
| STJ70514 | St John's Laboratory | 100 µg | EUR 430.8 |
Anti-Hsp40/DNAJB1 Antibody |
|||
| PA1043 | BosterBio | 100ug/vial | EUR 400.8 |
Dnajb14 sgRNA CRISPR Lentivector set (Rat) |
|||
| K6216401 | ABM | 3 x 1.0 ug | EUR 406.8 |
Dnajb14 3'UTR GFP Stable Cell Line |
|||
| TU155250 | ABM | 1.0 ml | Ask for price |
DNAJB14 3'UTR GFP Stable Cell Line |
|||
| TU056138 | ABM | 1.0 ml | EUR 2799.6 |
Dnajb14 3'UTR GFP Stable Cell Line |
|||
| TU253509 | ABM | 1.0 ml | Ask for price |
Dnajb14 sgRNA CRISPR Lentivector set (Mouse) |
|||
| K4586901 | ABM | 3 x 1.0 ug | EUR 406.8 |
DNAJB14 sgRNA CRISPR Lentivector set (Human) |
|||
| K0614501 | ABM | 3 x 1.0 ug | EUR 406.8 |
DNAJB14 Protein Vector (Rat) (pPM-C-HA) |
|||
| PV264404 | ABM | 500 ng | EUR 723.6 |
DNAJB14 Protein Vector (Rat) (pPB-C-His) |
|||
| PV264402 | ABM | 500 ng | EUR 723.6 |
DNAJB14 Protein Vector (Rat) (pPB-N-His) |
|||
| PV264403 | ABM | 500 ng | EUR 723.6 |
DNAJB14 Protein Vector (Rat) (pPM-C-His) |
|||
| PV264405 | ABM | 500 ng | EUR 723.6 |
DNAJB14 Protein Vector (Human) (pPM-C-HA) |
|||
| PV012707 | ABM | 500 ng | EUR 394.8 |
DNAJB14 Protein Vector (Mouse) (pPM-C-HA) |
|||
| PV172640 | ABM | 500 ng | EUR 723.6 |
DNAJB14 Protein Vector (Human) (pPB-C-His) |
|||
| PV012705 | ABM | 500 ng | EUR 394.8 |
DNAJB14 Protein Vector (Human) (pPB-N-His) |
|||
| PV012706 | ABM | 500 ng | EUR 394.8 |
DNAJB14 Protein Vector (Human) (pPM-C-His) |
|||
| PV012708 | ABM | 500 ng | EUR 394.8 |
DNAJB14 Protein Vector (Mouse) (pPB-C-His) |
|||
| PV172638 | ABM | 500 ng | EUR 723.6 |
DNAJB14 Protein Vector (Mouse) (pPB-N-His) |
|||
| PV172639 | ABM | 500 ng | EUR 723.6 |
DNAJB14 Protein Vector (Mouse) (pPM-C-His) |
|||
| PV172641 | ABM | 500 ng | EUR 723.6 |
DNAJB14 3'UTR Luciferase Stable Cell Line |
|||
| TU006138 | ABM | 1.0 ml | EUR 2799.6 |
Dnajb14 3'UTR Luciferase Stable Cell Line |
|||
| TU105250 | ABM | 1.0 ml | Ask for price |
Dnajb14 3'UTR Luciferase Stable Cell Line |
|||
| TU203509 | ABM | 1.0 ml | Ask for price |
Dnajb14 sgRNA CRISPR Lentivector (Rat) (Target 1) |
|||
| K6216402 | ABM | 1.0 ug DNA | EUR 184.8 |
Dnajb14 sgRNA CRISPR Lentivector (Rat) (Target 2) |
|||
| K6216403 | ABM | 1.0 ug DNA | EUR 184.8 |
Dnajb14 sgRNA CRISPR Lentivector (Rat) (Target 3) |
|||
| K6216404 | ABM | 1.0 ug DNA | EUR 184.8 |
Dnajb14 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
|||
| K4586902 | ABM | 1.0 ug DNA | EUR 184.8 |
Dnajb14 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
|||
| K4586903 | ABM | 1.0 ug DNA | EUR 184.8 |
Dnajb14 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
|||
| K4586904 | ABM | 1.0 ug DNA | EUR 184.8 |
DNAJB14 sgRNA CRISPR Lentivector (Human) (Target 1) |
|||
| K0614502 | ABM | 1.0 ug DNA | EUR 184.8 |
DNAJB14 sgRNA CRISPR Lentivector (Human) (Target 2) |
|||
| K0614503 | ABM | 1.0 ug DNA | EUR 184.8 |
DNAJB14 sgRNA CRISPR Lentivector (Human) (Target 3) |
|||
| K0614504 | ABM | 1.0 ug DNA | EUR 184.8 |
DNAJB11 antibody |
|||
| 70R-16873 | Fitzgerald | 50 ul | EUR 522 |
|
Description: Rabbit polyclonal DNAJB11 antibody |
|||
DNAJB12 antibody |
|||
| 70R-16874 | Fitzgerald | 50 ul | EUR 522 |
|
Description: Rabbit polyclonal DNAJB12 antibody |
|||
DNAJB12 antibody |
|||
| 70R-6342 | Fitzgerald | 50 ug | EUR 560.4 |
|
Description: Rabbit polyclonal DNAJB12 antibody |
|||
DNAJB11 antibody |
|||
| 70R-7353 | Fitzgerald | 50 ug | EUR 560.4 |
|
Description: Rabbit polyclonal DNAJB11 antibody |
|||
DNAJB11 antibody |
|||
| 70R-51002 | Fitzgerald | 100 ul | EUR 292.8 |
|
Description: Purified Polyclonal DNAJB11 antibody |
|||
DNAJB11 antibody |
|||
| 70R-35398 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Purified Rabbit polyclonal DNAJB11 antibody |
|||
DNAJB11 Antibody |
|||
| 34657-100ul | SAB | 100ul | EUR 302.4 |
DNAJB11 Antibody |
|||
| 34657-50ul | SAB | 50ul | EUR 224.4 |
DNAJB11 Antibody |
|||
| ABD4010 | Lifescience Market | 100 ug | EUR 525.6 |
DNAJB12 Antibody |
|||
| DF12598 | Affbiotech | 200ul | EUR 420 |
DNAJB11 Antibody |
|||
| DF4010 | Affbiotech | 200ul | EUR 420 |
DNAJB11 Antibody |
|||
| CSB-PA033940- | Cusabio | each | EUR 402 |
|
Description: A polyclonal antibody against DNAJB11. Recognizes DNAJB11 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100 |
|||
DNAJB11 Antibody |
|||
| CSB-PA033940-100ul | Cusabio | 100ul | EUR 379.2 |
|
Description: A polyclonal antibody against DNAJB11. Recognizes DNAJB11 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100 |
|||
DNAJB11 Antibody |
|||
| 1-CSB-PA890663LA01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against DNAJB11. Recognizes DNAJB11 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200 |
|||
DNAJB11 Antibody |
|||
| 1-CSB-PA007004GA01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against DNAJB11. Recognizes DNAJB11 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF |
|||
DNAJB12 Antibody |
|||
| 1-CSB-PA007005GA01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against DNAJB12. Recognizes DNAJB12 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
|||
DNAJB11 Antibody |
|||
| 1-CSB-PA002411 | Cusabio |
|
|
|
Description: A polyclonal antibody against DNAJB11. Recognizes DNAJB11 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000 |
|||
DNAJB1 antibody |
|||
| 10R-3856 | Fitzgerald | 100 ul | EUR 829.2 |
|
Description: Mouse monoclonal DNAJB1 antibody |
|||
DNAJB1 antibody |
|||
| 10R-3857 | Fitzgerald | 100 ul | EUR 829.2 |
|
Description: Mouse monoclonal DNAJB1 antibody |
|||
DNAJB1 antibody |
|||
| 10R-3858 | Fitzgerald | 100 ul | EUR 829.2 |
|
Description: Mouse monoclonal DNAJB1 antibody |
|||
DNAJB2 antibody |
|||
| 10R-3859 | Fitzgerald | 100 ul | EUR 829.2 |
|
Description: Mouse monoclonal DNAJB2 antibody |
|||
DNAJB2 antibody |
|||
| 10R-3860 | Fitzgerald | 100 ul | EUR 871.2 |
|
Description: Mouse monoclonal DNAJB2 antibody |
|||
DNAJB2 antibody |
|||
| 22071-100ul | SAB | 100ul | EUR 468 |
DNAJB6 antibody |
|||
| 22547-100ul | SAB | 100ul | EUR 468 |
DNAJB5 antibody |
|||
| 20R-1311 | Fitzgerald | 100 ug | EUR 452.4 |
|
Description: Rabbit polyclonal DNAJB5 antibody |
|||
DNAJB9 Antibody |
|||
| 42984-100ul | SAB | 100ul | EUR 302.4 |
DNAJB6 antibody |
|||
| 70R-2575 | Fitzgerald | 50 ug | EUR 560.4 |
|
Description: Rabbit polyclonal DNAJB6 antibody |
|||
DNAJB4 antibody |
|||
| 70R-9161 | Fitzgerald | 50 ug | EUR 560.4 |
|
Description: Affinity purified rabbit polyclonal DNAJB4 antibody |
|||
DNAJB1 antibody |
|||
| 70R-16872 | Fitzgerald | 50 ul | EUR 522 |
|
Description: Rabbit polyclonal DNAJB1 antibody |
|||
DNAJB2 antibody |
|||
| 70R-16876 | Fitzgerald | 50 ul | EUR 522 |
|
Description: Rabbit polyclonal DNAJB2 antibody |
|||
DNAJB3 antibody |
|||
| 70R-16877 | Fitzgerald | 50 ul | EUR 522 |
|
Description: Rabbit polyclonal DNAJB3 antibody |
|||
DNAJB4 antibody |
|||
| 70R-16878 | Fitzgerald | 50 ul | EUR 522 |
|
Description: Rabbit polyclonal DNAJB4 antibody |
|||
DNAJB5 antibody |
|||
| 70R-16879 | Fitzgerald | 50 ul | EUR 522 |
|
Description: Rabbit polyclonal DNAJB5 antibody |
|||
DNAJB6 antibody |
|||
| 70R-16880 | Fitzgerald | 50 ul | EUR 522 |
|
Description: Rabbit polyclonal DNAJB6 antibody |
|||
DNAJB8 antibody |
|||
| 70R-16881 | Fitzgerald | 50 ul | EUR 522 |
|
Description: Rabbit polyclonal DNAJB8 antibody |
|||
DNAJB6 antibody |
|||
| 70R-12627 | Fitzgerald | 100 ul | EUR 548.4 |
|
Description: Affinity purified Rabbit polyclonal DNAJB6 antibody |
|||
DNAJB2 antibody |
|||
| 70R-12806 | Fitzgerald | 100 ul | EUR 548.4 |
|
Description: Affinity purified Rabbit polyclonal DNAJB2 antibody |
|||
DNAJB9 antibody |
|||
| 70R-7401 | Fitzgerald | 50 ug | EUR 560.4 |
|
Description: Rabbit polyclonal DNAJB9 antibody |
|||
DNAJB4 antibody |
|||
| 70R-32430 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal DNAJB4 antibody |
|||
DNAJB1 antibody |
|||
| 70R-3606 | Fitzgerald | 50 ug | EUR 560.4 |
|
Description: Rabbit polyclonal DNAJB1 antibody |
|||
DNAJB4 Antibody |
|||
| 34246-100ul | SAB | 100ul | EUR 302.4 |
DNAJB4 Antibody |
|||
| 34246-50ul | SAB | 50ul | EUR 224.4 |
DNAJB1 Antibody |
|||
| 32894-100ul | SAB | 100ul | EUR 302.4 |
DNAJB4 Antibody |
|||
| 47067-100ul | SAB | 100ul | EUR 302.4 |
DNAJB5 Antibody |
|||
| 45605-100ul | SAB | 100ul | EUR 302.4 |
DNAJB5 Antibody |
|||
| 45605-50ul | SAB | 50ul | EUR 224.4 |
DNAJB2 Antibody |
|||
| ABD10101 | Lifescience Market | 100 ug | EUR 525.6 |
DNAJB6 Antibody |
|||
| ABD2489 | Lifescience Market | 100 ug | EUR 525.6 |
DNAJB4 Antibody |
|||
| ABD3594 | Lifescience Market | 100 ug | EUR 525.6 |
DNAJB5 Antibody |
|||
| ABD8941 | Lifescience Market | 100 ug | EUR 525.6 |
DNAJB1 Antibody |
|||
| ABD7382 | Lifescience Market | 100 ug | EUR 525.6 |
DNAJB2 Antibody |
|||
| DF10101 | Affbiotech | 200ul | EUR 420 |
DNAJB3 Antibody |
|||
| DF12268 | Affbiotech | 200ul | EUR 420 |
DNAJB5 Antibody |
|||
| DF8941 | Affbiotech | 200ul | EUR 420 |
DNAJB6 Antibody |
|||
| DF2489 | Affbiotech | 200ul | EUR 420 |
DNAJB4 Antibody |
|||
| DF3594 | Affbiotech | 200ul | EUR 420 |
DNAJB6 Antibody |
|||
| 1-CSB-PA03239A0Rb | Cusabio |
|
|
|
Description: A polyclonal antibody against DNAJB6. Recognizes DNAJB6 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF; Recommended dilution: WB:1:2000-1:10000, IF:1:50-1:200 |
|||
DNAJB1 Antibody |
|||
| 1-CSB-PA03934A0Rb | Cusabio |
|
|
|
Description: A polyclonal antibody against DNAJB1. Recognizes DNAJB1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
|||
DNAJB1 Antibody |
|||
| 1-CSB-PA183145 | Cusabio |
|
|
|
Description: A polyclonal antibody against DNAJB1. Recognizes DNAJB1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:50-1:200 |
|||
DNAJB4 Antibody |
|||
| CSB-PA185290- | Cusabio | each | EUR 402 |
|
Description: A polyclonal antibody against DNAJB4. Recognizes DNAJB4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
|||
DNAJB4 Antibody |
|||
| CSB-PA185290-100ul | Cusabio | 100ul | EUR 379.2 |
|
Description: A polyclonal antibody against DNAJB4. Recognizes DNAJB4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
|||
DNAJB4 Antibody |
|||
| 1-CSB-PA10929A0Rb | Cusabio |
|
|
|
Description: A polyclonal antibody against DNAJB4. Recognizes DNAJB4 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200 |
|||
DNAJB1 Antibody |
|||
| CSB-PA288820- | Cusabio | each | EUR 402 |
|
Description: A polyclonal antibody against DNAJB1. Recognizes DNAJB1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
|||
DNAJB1 Antibody |
|||
| CSB-PA288820-100ul | Cusabio | 100ul | EUR 379.2 |
|
Description: A polyclonal antibody against DNAJB1. Recognizes DNAJB1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
|||
DNAJB8 Antibody |
|||
| 1-CSB-PA836779LA01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against DNAJB8. Recognizes DNAJB8 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200 |
|||
DNAJB9 Antibody |
|||
| 1-CSB-PA866205ESR1HU | Cusabio |
|
|
|
Description: A polyclonal antibody against DNAJB9. Recognizes DNAJB9 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
|||
DNAJB1 Antibody |
|||
| 1-CSB-PA795084 | Cusabio |
|
|
|
Description: A polyclonal antibody against DNAJB1. Recognizes DNAJB1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100 |
|||
DNAJB1 Antibody |
|||
| 1-CSB-PA700713 | Cusabio |
|
|
|
Description: A polyclonal antibody against DNAJB1. Recognizes DNAJB1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC-p:1:100-1:300.ELISA:1/10000 |
|||