Overview
Product name
Luxol Fast Blue staining kit (myelin staining)
Product description
The Ab150675 Luxol Fast Blue Staining Kit (Myelin Stain) is designed to stain myelin / myelinated axons and Nissl bodies in formalin-fixed and paraffin-embedded tissue as well as frozen tissue. This product is used to identify the basic neural structure in sections of the brain or spinal cord.
Summary of Luxol Fast Blue staining protocol:
- deparaffinized the sections if necessary and hydrate in water
- incubate in Luxol Fast Blue solution for 24 hours at room temperature or 2 hours at 60ºC
- rinse with water
- differentiate by immersing it in a lithium carbonate solution
- differentiate even more by dipping in alcohol reagent
- rinse with water
- incubate in Cresyl Echt Violet for 2-5 min
- rinse with water
- dehydrate in absolute alcohol
- clear and mount
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and assembly in the IHC kits and reagents guide.
Relevance
Myelin is produced by oligodendrocytes in the CNS and Schwann cells in the peripheral nervous system (PNS). Defining the differences between CNS myelin and PNS can provide information on the myelination process and pathogenesis of certain idiopathic diseases involving myelin, such as multiple sclerosis, certain leukodystrophies, and other myelinopathies.
Myelin is made up of approximately 80% lipid fat and approximately 20% protein. Some of the proteins that make up myelin are myelin basic protein (MBP), myelin oligodendrocyte glycoprotein (MOG), and proteolipid protein (PLP). Myelin is mainly made up of a glycolipid called galactocerebroside. The entanglement of the hydrocarbon chains of sphingomyelin serves to strengthen the myelin sheath.
Cell location
Integral membrane protein.
Alternative names
- LITTLE CAT
- MOG IG 2
- Myelin oligodendrocyte glycoprotein